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The main advantage of in situ treatment is that it allows ground water to be treated without being brought to the surface, resulting in significant cost savings. In situ treatment, however, generally requires longer time periods, and there is less certainty about the uniformity of treatment because of the variability in aquifer characteristics and because the efficacy of the process is more difficult to verify.

Bioremediation techniques are destruction techniques directed toward stimulating the microorganisms to grow and use the contaminants as a food and energy source by creating a favorable environment for the microorganisms. Generally, this means providing some combination of oxygen, nutrients, and moisture, and controlling the temperature and pH. Sometimes, microorganisms adapted for degradation of the specific contaminants are applied to enhance the process.

Biological processes are typically implemented at low cost. Contaminants are destroyed and little to no residual treatment is required. Some compounds, however, may be broken down into more toxic by-products during the bioremediation process (e.g., TCE to vinyl chloride). In in situ applications, these by-products may be mobilized in ground water if no control techniques are used. Typically, to address this issue, bioremediation will be performed above a low permeability soil layer and with ground water monitoring wells downgradient of the remediation area. This type of treatment scheme requires aquifer and contaminant characterization and may still require extracted groundwater treatment.

Although not all organic compounds are amenable to biodegradation, bioremediation techniques have been successfully used to remediate ground water contaminated by petroleum hydrocarbons, solvents, pesticides, wood preservatives, and other organic chemicals. Bioremediation has no expected effect on inorganic contaminants.

A section from a FRTR report and original working URL: https://frtr.gov/matrix2/section3/3_9.html 

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